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Cellular Technology Ltd color elispot 96 well plate white
Color Elispot 96 Well Plate White, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 96/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/color+elispot+96+well+plate+white/96-Well+White/pmc10034440-52-11-5
Average 96 stars, based on 4 article reviews

color elispot 96 well plate white - by Bioz Stars, 2026-08

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Figure 1. Most—but not all—healthy donors demonstrate preexisting T-cell immune memory to phosphopeptides displayed on cancer cells. Summary response data for CD8þCD45ROþ T cells from healthy donors (HD) stimulated once in vitro with peptide-pulsed autologous mDCs and cultured for 14 days, measured in triplicate wells in an IFNg ELISpot assay. Data represent a minimum of 3 experiments for each peptide to which a response was observed in a donor. Peptides to which responses were not observed initially may have been analyzed in fewer experiments. A, Responses of HLA-A2þ donors to the indicated HLA-A2–restricted peptides. B, Responses of HLA-B7þ donors to HLA-B7–restricted peptides. The bottom portion of B presents data for 5 HLA-B7–restricted phosphopeptides that were analyzed for HD43,HD44, HD67, and HD89 because of limited sample availability. Insufﬁcient PBMCs were available from HD67 and HD89 to assess responses against the larger cohort of HLA-B7–restricted phosphopeptides.

Journal: Cancer Immunology Research

Article Title: Characteristics of immune memory and effector activity to cancer-expressed MHC class I phosphopeptides differ in healthy donors and ovarian cancer patients

doi: 10.1158/2326-6066.cir-21-0111

Figure Lengend Snippet: Figure 1. Most—but not all—healthy donors demonstrate preexisting T-cell immune memory to phosphopeptides displayed on cancer cells. Summary response data for CD8þCD45ROþ T cells from healthy donors (HD) stimulated once in vitro with peptide-pulsed autologous mDCs and cultured for 14 days, measured in triplicate wells in an IFNg ELISpot assay. Data represent a minimum of 3 experiments for each peptide to which a response was observed in a donor. Peptides to which responses were not observed initially may have been analyzed in fewer experiments. A, Responses of HLA-A2þ donors to the indicated HLA-A2–restricted peptides. B, Responses of HLA-B7þ donors to HLA-B7–restricted peptides. The bottom portion of B presents data for 5 HLA-B7–restricted phosphopeptides that were analyzed for HD43,HD44, HD67, and HD89 because of limited sample availability. Insufﬁcient PBMCs were available from HD67 and HD89 to assess responses against the larger cohort of HLA-B7–restricted phosphopeptides.

Article Snippet: IFNg Single-Color ELISpot Plates (Cellular Technology Limited; cat #hIFNg-1M/2) were activated with 70% ethanol and incubated with primary antibody at 4 C overnight.

Techniques: In Vitro, Cell Culture, Enzyme-linked Immunospot

Figure 2. T-cell responses to viral epitopes deﬁne two response patterns that distinguish recent or ongoing antigen exposure. A–D, The indicated CD8þ T-cell subsets were enriched by cell sorting, and analyzed in an IFNg ELISpot assay either after one in vitro stimulation with the indicated viral peptide–pulsed autologous DCs and a 14- day culture (cultured), or immediately ex vivo (direct). Responses were normalized for the expansion of cultured cells over 14 days and CD8þ T-cell subset percentages in the donor’s blood, as described in Materials and Methods. Responses in each subset are reported both as the number of IFNgþ cells per 106 CD8þ T cells (left y-axis) and the subset percentage of the total measured response (right y-axis). Responses to inﬂuenza M1 were measured in PBMCs collected before the donor received the annual ﬂu vaccine and in the absence of illness (pre-vaccine) or 16 to 17 days after receiving an inﬂuenza vaccine (post-vaccine). C, Due to low cell numbers, no data are available for TSCM responses in HD44. The color coding for each subset is based on Supplementary Fig. S1. Most plots are representative of more than 1 experiment, except HD44 cultured and direct responses to EBV BBLF2/3, HD44 cultured response to CMV pp65495–503, HD43 direct response to inﬂuenza pre- vaccine, and cultured and direct response post-vaccine. HD, healthy donor.

Journal: Cancer Immunology Research

Article Title: Characteristics of immune memory and effector activity to cancer-expressed MHC class I phosphopeptides differ in healthy donors and ovarian cancer patients

doi: 10.1158/2326-6066.cir-21-0111

Figure Lengend Snippet: Figure 2. T-cell responses to viral epitopes deﬁne two response patterns that distinguish recent or ongoing antigen exposure. A–D, The indicated CD8þ T-cell subsets were enriched by cell sorting, and analyzed in an IFNg ELISpot assay either after one in vitro stimulation with the indicated viral peptide–pulsed autologous DCs and a 14- day culture (cultured), or immediately ex vivo (direct). Responses were normalized for the expansion of cultured cells over 14 days and CD8þ T-cell subset percentages in the donor’s blood, as described in Materials and Methods. Responses in each subset are reported both as the number of IFNgþ cells per 106 CD8þ T cells (left y-axis) and the subset percentage of the total measured response (right y-axis). Responses to inﬂuenza M1 were measured in PBMCs collected before the donor received the annual ﬂu vaccine and in the absence of illness (pre-vaccine) or 16 to 17 days after receiving an inﬂuenza vaccine (post-vaccine). C, Due to low cell numbers, no data are available for TSCM responses in HD44. The color coding for each subset is based on Supplementary Fig. S1. Most plots are representative of more than 1 experiment, except HD44 cultured and direct responses to EBV BBLF2/3, HD44 cultured response to CMV pp65495–503, HD43 direct response to inﬂuenza pre- vaccine, and cultured and direct response post-vaccine. HD, healthy donor.

Article Snippet: IFNg Single-Color ELISpot Plates (Cellular Technology Limited; cat #hIFNg-1M/2) were activated with 70% ethanol and incubated with primary antibody at 4 C overnight.

Techniques: FACS, Enzyme-linked Immunospot, In Vitro, Cell Culture, Ex Vivo

Figure 3. Longitudinal cultured response patterns to phosphopeptides by healthy donor 44 (HD44). A and B, CD8þ T-cell subsets isolated by FACS from blood samples collected at different time points were stimulated once in vitro with peptide-pulsed autologous mDCs and cultured for 14 days, and then responses to the indicated phosphopeptides were measured in triplicate wells using an IFNg ELISpot assay. Responses were normalized for the expansion of cultured cells and subset percentages in the donor’s blood, as described in Materials and Methods. Responses in each subset are reported both as the number of IFNgþ cells per 106 CD8þ T cells (left y-axis) and the subset percentage of the total measured response (right y-axis). The color coding for each subset is based on Supplementary Fig. S1. Each time point reﬂects one analysis of PBMCs harvested at that time point. A, Longitudinal responses measured in the ﬁrst collected blood sample (initial) and in samples collected at the indicated times in relation to the initial sample. B, The N þ SCM subset was sorted using CD95 to evaluate responses from na€ve or memory stem cells. Responses shown are from 5 months (pWWTR186–94), 10 months (pPEG10248–259 and pPEG10248–258), or 13 months (pLSP1249–258, pCHEK1461–471, pSRP72466–473, and pCDC25B38–46) and are representative of at least 2 examined time points. C, Responses to the peptides in each box were considered related to Response Pattern 2 and evidence of active immunogenic exposure at the indicated time points.

Journal: Cancer Immunology Research

Article Title: Characteristics of immune memory and effector activity to cancer-expressed MHC class I phosphopeptides differ in healthy donors and ovarian cancer patients

doi: 10.1158/2326-6066.cir-21-0111

Figure Lengend Snippet: Figure 3. Longitudinal cultured response patterns to phosphopeptides by healthy donor 44 (HD44). A and B, CD8þ T-cell subsets isolated by FACS from blood samples collected at different time points were stimulated once in vitro with peptide-pulsed autologous mDCs and cultured for 14 days, and then responses to the indicated phosphopeptides were measured in triplicate wells using an IFNg ELISpot assay. Responses were normalized for the expansion of cultured cells and subset percentages in the donor’s blood, as described in Materials and Methods. Responses in each subset are reported both as the number of IFNgþ cells per 106 CD8þ T cells (left y-axis) and the subset percentage of the total measured response (right y-axis). The color coding for each subset is based on Supplementary Fig. S1. Each time point reﬂects one analysis of PBMCs harvested at that time point. A, Longitudinal responses measured in the ﬁrst collected blood sample (initial) and in samples collected at the indicated times in relation to the initial sample. B, The N þ SCM subset was sorted using CD95 to evaluate responses from na€ve or memory stem cells. Responses shown are from 5 months (pWWTR186–94), 10 months (pPEG10248–259 and pPEG10248–258), or 13 months (pLSP1249–258, pCHEK1461–471, pSRP72466–473, and pCDC25B38–46) and are representative of at least 2 examined time points. C, Responses to the peptides in each box were considered related to Response Pattern 2 and evidence of active immunogenic exposure at the indicated time points.

Article Snippet: IFNg Single-Color ELISpot Plates (Cellular Technology Limited; cat #hIFNg-1M/2) were activated with 70% ethanol and incubated with primary antibody at 4 C overnight.

Techniques: Cell Culture, Isolation, In Vitro, Enzyme-linked Immunospot

Figure 4. Longitudinal cultured response patterns to phosphopeptides by healthy donor 43 (HD43). A and B, CD8þ T-cell subsets isolated by FACS from blood samples collected at different time points were stimulated once in vitro with peptide-pulsed autologous mDCs and cultured for 14 days, and then responses to the indicated phosphopeptides were measured in triplicate wells using an IFNg ELISpot assay. Responses were normalized for the expansion of cultured cells and subset percentages in the donor’s blood, as described in Materials and Methods. Responses in each subset are reported both as the number of IFNgþ cells per 106 CD8þ T cells (left y-axis) and the subset percentage of the total measured response (right y-axis). The color coding for each subset is based on Supplementary Fig. S1. Each time point reﬂects one analysis of PBMCs harvested at that time point. A, Longitudinal responses measured in the ﬁrst collected blood sample (initial) and in samples collected at the indicated times in relation to the initial sample. B, The N þ SCM subset was sorted using CD95 to evaluate responses from na€ve or memory stem cells. Responses shown are from 9 months (pPEG10248–259), 13 months (pCDC25B38–46), or 16 months (pCHEK1461–471 and pSRP72466–473) and are representative of at least 2 examined time points. C, Responses to the peptides in each box were considered related to Response Pattern 2 and evidence of active immunogenicexposure atthe indicated time points.

Journal: Cancer Immunology Research

Article Title: Characteristics of immune memory and effector activity to cancer-expressed MHC class I phosphopeptides differ in healthy donors and ovarian cancer patients

doi: 10.1158/2326-6066.cir-21-0111

Figure Lengend Snippet: Figure 4. Longitudinal cultured response patterns to phosphopeptides by healthy donor 43 (HD43). A and B, CD8þ T-cell subsets isolated by FACS from blood samples collected at different time points were stimulated once in vitro with peptide-pulsed autologous mDCs and cultured for 14 days, and then responses to the indicated phosphopeptides were measured in triplicate wells using an IFNg ELISpot assay. Responses were normalized for the expansion of cultured cells and subset percentages in the donor’s blood, as described in Materials and Methods. Responses in each subset are reported both as the number of IFNgþ cells per 106 CD8þ T cells (left y-axis) and the subset percentage of the total measured response (right y-axis). The color coding for each subset is based on Supplementary Fig. S1. Each time point reﬂects one analysis of PBMCs harvested at that time point. A, Longitudinal responses measured in the ﬁrst collected blood sample (initial) and in samples collected at the indicated times in relation to the initial sample. B, The N þ SCM subset was sorted using CD95 to evaluate responses from na€ve or memory stem cells. Responses shown are from 9 months (pPEG10248–259), 13 months (pCDC25B38–46), or 16 months (pCHEK1461–471 and pSRP72466–473) and are representative of at least 2 examined time points. C, Responses to the peptides in each box were considered related to Response Pattern 2 and evidence of active immunogenicexposure atthe indicated time points.

Article Snippet: IFNg Single-Color ELISpot Plates (Cellular Technology Limited; cat #hIFNg-1M/2) were activated with 70% ethanol and incubated with primary antibody at 4 C overnight.

Techniques: Cell Culture, Isolation, In Vitro, Enzyme-linked Immunospot

Figure 5. Direct responses to phosphopeptides in healthy donors (HD) are largely consistent with cultured response patterns. Responses of CD8þ T-cell subsets isolated by FACS from HD44 (A) and HD43 (B) to the indicated phos- phopeptides were measured directly ex vivo and normalized for the subset percentages in the donor’s blood, as described in Materials and Methods. Responses in each subset are reported both as the number of IFNgþ cells per 106 CD8þ T cells (left y-axis) and the subset percentage of the total measured response (right y-axis). The color coding for each subset is based on Supplementary Fig. S1. Each time point reﬂects one analysis of PBMCs harvested at that time point.

Journal: Cancer Immunology Research

Article Title: Characteristics of immune memory and effector activity to cancer-expressed MHC class I phosphopeptides differ in healthy donors and ovarian cancer patients

doi: 10.1158/2326-6066.cir-21-0111

Figure Lengend Snippet: Figure 5. Direct responses to phosphopeptides in healthy donors (HD) are largely consistent with cultured response patterns. Responses of CD8þ T-cell subsets isolated by FACS from HD44 (A) and HD43 (B) to the indicated phos- phopeptides were measured directly ex vivo and normalized for the subset percentages in the donor’s blood, as described in Materials and Methods. Responses in each subset are reported both as the number of IFNgþ cells per 106 CD8þ T cells (left y-axis) and the subset percentage of the total measured response (right y-axis). The color coding for each subset is based on Supplementary Fig. S1. Each time point reﬂects one analysis of PBMCs harvested at that time point.

Article Snippet: IFNg Single-Color ELISpot Plates (Cellular Technology Limited; cat #hIFNg-1M/2) were activated with 70% ethanol and incubated with primary antibody at 4 C overnight.

Techniques: Cell Culture, Isolation, Ex Vivo

Figure 6. Immune responses to viral and phosphorylated peptides by ovarian cancer patients. CD8þ T-cell subsets (TCMþ TN and TEM þ TEMRA þ TSCM; Supplementary Fig. S2) isolated by FACS from 4 identiﬁed ovarian cancer patients (Sup- plementary Table S3) were stimulated once in vitro with the indicated viral or phosphorylated peptide–pulsed autologous mDCs and irradiated CD4CD8 PBLs as antigen-presenting cells and cultured for 14 days, and then responses to the same peptides were measured in triplicate wells in an IFNg ELISpot assay. Responses were normalized for the expansion of cultured cells and subset percentages in the donor’s blood, as described in Materi- als and Methods. Each datapoint reﬂects one analysis of PBMCs or TILs harvested at the identiﬁed time point, and is the sum of responses from both sorted subsets. T, phosphopeptide expressed on the patient’s tumor as identiﬁed by mass spectrometry.

Journal: Cancer Immunology Research

Article Title: Characteristics of immune memory and effector activity to cancer-expressed MHC class I phosphopeptides differ in healthy donors and ovarian cancer patients

doi: 10.1158/2326-6066.cir-21-0111

Figure Lengend Snippet: Figure 6. Immune responses to viral and phosphorylated peptides by ovarian cancer patients. CD8þ T-cell subsets (TCMþ TN and TEM þ TEMRA þ TSCM; Supplementary Fig. S2) isolated by FACS from 4 identiﬁed ovarian cancer patients (Sup- plementary Table S3) were stimulated once in vitro with the indicated viral or phosphorylated peptide–pulsed autologous mDCs and irradiated CD4CD8 PBLs as antigen-presenting cells and cultured for 14 days, and then responses to the same peptides were measured in triplicate wells in an IFNg ELISpot assay. Responses were normalized for the expansion of cultured cells and subset percentages in the donor’s blood, as described in Materi- als and Methods. Each datapoint reﬂects one analysis of PBMCs or TILs harvested at the identiﬁed time point, and is the sum of responses from both sorted subsets. T, phosphopeptide expressed on the patient’s tumor as identiﬁed by mass spectrometry.

Article Snippet: IFNg Single-Color ELISpot Plates (Cellular Technology Limited; cat #hIFNg-1M/2) were activated with 70% ethanol and incubated with primary antibody at 4 C overnight.

Techniques: Isolation, In Vitro, Irradiation, Cell Culture, Enzyme-linked Immunospot, Phospho-proteomics, Mass Spectrometry

Figure 7. Phosphopeptide expression on patient tumor is associated with an active immune response. CD8þ T-cell subsets (TCM þ TN and TEM þ TEMRA þ TSCM; Supplementary Fig. S2) isolated by FACS from ovarian cancer patients VTB241 (A) and VTB239 (B; Supplementary Table S3) were stimulated once in vitro with the indicated viral or phosphorylated peptide–pulsed autologous mDCs and irradiated CD4CD8 PBLs as antigen-presenting cells and cultured for 14 days, and then responses to the same peptides were measured in triplicate wells in an IFNg ELISpot assay. Responses were normalized for the expansion of cultured cells and subset percentages in the donor’s blood, as described in Materials and Methods. Responses in each subset are reported both as the number of IFNgþ cells per 106 CD8þ T cells (left y-axis) and the subset percentage of the total measured response (right y-axis). The color coding for each subset is based on Supplementary Fig. S2. Each graph represents one analysis of PBLs or TILs.

Journal: Cancer Immunology Research

Article Title: Characteristics of immune memory and effector activity to cancer-expressed MHC class I phosphopeptides differ in healthy donors and ovarian cancer patients

doi: 10.1158/2326-6066.cir-21-0111

Figure Lengend Snippet: Figure 7. Phosphopeptide expression on patient tumor is associated with an active immune response. CD8þ T-cell subsets (TCM þ TN and TEM þ TEMRA þ TSCM; Supplementary Fig. S2) isolated by FACS from ovarian cancer patients VTB241 (A) and VTB239 (B; Supplementary Table S3) were stimulated once in vitro with the indicated viral or phosphorylated peptide–pulsed autologous mDCs and irradiated CD4CD8 PBLs as antigen-presenting cells and cultured for 14 days, and then responses to the same peptides were measured in triplicate wells in an IFNg ELISpot assay. Responses were normalized for the expansion of cultured cells and subset percentages in the donor’s blood, as described in Materials and Methods. Responses in each subset are reported both as the number of IFNgþ cells per 106 CD8þ T cells (left y-axis) and the subset percentage of the total measured response (right y-axis). The color coding for each subset is based on Supplementary Fig. S2. Each graph represents one analysis of PBLs or TILs.

Article Snippet: IFNg Single-Color ELISpot Plates (Cellular Technology Limited; cat #hIFNg-1M/2) were activated with 70% ethanol and incubated with primary antibody at 4 C overnight.

Techniques: Phospho-proteomics, Expressing, Isolation, In Vitro, Irradiation, Cell Culture, Enzyme-linked Immunospot

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